Explain how bacteria, archaea, and eukaryotes are distinguished from one another at the domain level of classification.

IB DP Biology Standard Level (2023 syllabus) — A3.1 Diversity of organisms · Explain · 4 marks · View as Markdown

Written & reviewed by James Millett — Biology (Imperial College London), PGCE Science (University of Cambridge).

Model answer (4 marks)

Bacteria and archaea are both prokaryotes, lacking a membrane‑bound nucleus, whereas eukaryotes possess a nucleus.
Archaea differ from bacteria in their cell‑membrane lipids: archaea have ether‑linked lipids, while bacteria have ester‑linked lipids.
Bacterial cell walls contain peptidoglycan; archaea lack peptidoglycan.
Molecular data (e.g. rRNA gene sequences) show that archaea are more closely related to eukaryotes than to bacteria, supporting the three‑domain system.

Examiner tips

  • Use the exact terms – prokaryote, eukaryote, ether‑linked, ester‑linked, peptidoglycan, rRNA

Common mistakes

  • Confusing the presence of a nucleus with prokaryote/eukaryote distinction; mixing up lipid linkages; omitting the molecular evidence point

Mark scheme (4 marks)

  1. Bacteria and Archaea are both prokaryotes (lacking a membrane-bound nucleus), whereas Eukaryotes possess a membrane-bound nucleus.
  2. Archaea are distinguished from Bacteria by differences in their cell membrane composition, specifically the presence of ether-linked lipids in Archaea (compared to ester-linked lipids in Bacteria).
  3. Bacteria have peptidoglycan in their cell walls, whereas Archaea lack peptidoglycan.
  4. Molecular evidence (such as rRNA / ribosomal gene sequence comparisons) shows that Archaea are more closely related to Eukaryotes than to Bacteria, justifying three separate domains.

Key terms in this question

domain · Archaea

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