Explain how water acts as a metabolite in living organisms, giving two distinct examples of reactions in which water is either produced or consumed.

IB DP Biology Standard Level (2023 syllabus) — A1.1 Water · Explain · 4 marks · View as Markdown

Written & reviewed by James Millett — Biology (Imperial College London), PGCE Science (University of Cambridge).

Model answer (4 marks)

Water is a metabolite because it is directly involved as a reactant or product in metabolic reactions, not just as a solvent.
1. Hydrolysis – water is consumed. For example, the hydrolysis of a peptide bond in a polypeptide:
peptide + H₂O → two amino acids
or the hydrolysis of a triglyceride:
triglyceride + 3 H₂O → glycerol + 3 fatty acids.
2. Condensation – water is produced. For example, the formation of a peptide bond between two amino acids:
amino acid + amino acid → dipeptide + H₂O
or the synthesis of a glycosidic bond between two monosaccharides:
monosaccharide + monosaccharide → disaccharide + H₂O.
3. Photosynthesis – water is a reactant. In the light‑dependent reactions, water is split (photolysis) to give O₂, electrons and protons:
2 H₂O → 4 H⁺ + 4 e⁻ + O₂.
The electrons and protons are then used in the electron transport chain and to reduce NADP⁺ to NADPH.

Examiner tips

  • Use the word ‘metabolite’ to show understanding of the definition.
  • Give two clear examples – one where water is consumed, one where it is produced.
  • Mention the role of water in photosynthesis as a reactant.
  • Show the chemical equations where possible.

Mark scheme (4 marks)

  1. Water is a metabolite, meaning it participates directly as a reactant or product in metabolic reactions (not merely as a solvent).
  2. Water is consumed (used as a reactant) in hydrolysis reactions, e.g. the breakdown of polymers such as polypeptides, polysaccharides, or triglycerides into their monomers.
  3. Water is produced as a product in condensation reactions, e.g. formation of peptide bonds between amino acids, glycosidic bonds between monosaccharides, or ester bonds in lipid synthesis.
  4. Water is also a reactant in photosynthesis (light-dependent reactions / photolysis), where it is split to release oxygen, electrons, and protons / hydrogen ions that are used in the light-dependent stage.

Key terms in this question

metabolite

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