Distinguish between an outgroup and an ingroup in the context of cladistic analysis, and explain the role of the outgroup in identifying synapomorphies.

IB DP Biology Higher Level (2023 syllabus) — A3.2 Classification and cladistics · Distinguish / Explain · 4 marks · View as Markdown

Written & reviewed by James Millett — Biology (Imperial College London), PGCE Science (University of Cambridge).

Model answer (4 marks)

The ingroup consists of the taxa whose relationships are being studied.
The outgroup is a taxon that diverged earlier than the ingroup and is more distantly related to all ingroup members.
By comparing the ingroup to the outgroup we can recognise plesiomorphic states – traits shared with the outgroup are likely ancestral.
Synapomorphies are traits found in two or more ingroup members but absent in the outgroup, indicating shared derived character and defining a monophyletic clade.

Examiner tips

  • Use the words 'ingroup' and 'outgroup' explicitly; explain their relative positions in the tree.
  • Show that the outgroup provides a reference for ancestral states, then state how traits absent in the outgroup but present in the ingroup are synapomorphies.

Common mistakes

  • Confusing ingroup with outgroup or vice‑versa.
  • Failing to explain that the outgroup helps identify ancestral versus derived traits.

Mark scheme (4 marks)

  1. The ingroup comprises the taxa under study / the organisms whose evolutionary relationships are being investigated.
  2. The outgroup is a taxon that is more distantly related to all members of the ingroup / diverged from the ingroup before the ingroup taxa diverged from each other.
  3. Comparing the ingroup to the outgroup allows ancestral (plesiomorphic) character states to be identified, because traits shared between the outgroup and some ingroup members are likely ancestral.
  4. Synapomorphies (shared derived characters) are identified as traits present in two or more ingroup members but absent in the outgroup, indicating shared common ancestry within the ingroup / used to define monophyletic clades.

Key terms in this question

outgroup · ingroup

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