Describe how pectinase is used in the production of fruit juice, and explain why its use is beneficial to manufacturers.

Cambridge International IGCSE Biology (0610) — 21.2 Biotechnology · Describe · 4 marks · View as Markdown

Written & reviewed by James Millett — Biology (Imperial College London), PGCE Science (University of Cambridge).

Model answer (4 marks)

Pectinase is an enzyme that hydrolyses pectin, a polysaccharide that forms part of the plant cell wall.

In fruit juice production, pectinase is added to the fruit mash. The enzyme breaks down the pectin, weakening the cell walls and allowing the juice to flow out of the cells.

This increases the amount of juice that can be extracted from the same quantity of fruit, giving manufacturers a higher yield and a clearer product.

The higher yield means lower production costs and a more efficient use of raw materials, which is why manufacturers favour pectinase.

Examiner tips

  • Use the term ‘hydrolyses’ or ‘breaks down’ for pectinase action. Mention that pectin is part of the cell wall. Explain that breaking it down releases juice. Link the increased yield to cost‑effectiveness for manufacturers.

Common mistakes

  • Confusing pectinase with other enzymes such as amylase. Failing to state that pectin is in the cell wall. Not linking the higher yield to manufacturer benefits.

Mark scheme (4 marks)

  1. Pectinase breaks down pectin
  2. Pectin is found in / holds together plant cell walls
  3. Breaking down pectin releases the contents of the cells
  4. This increases the yield of fruit juice (produced)

Key terms in this question

pectinase · pectin

Related

More Biotechnology questions

▶ Try answering this question with AI marking (free) →